Journal article
Maintenance of the EBV-specific CD8 TCRαβ repertoire in immunosuppressed lung transplant recipients
TH Nguyen, NL Bird, EJ Grant, JJ Miles, PG Thomas, TC Kotsimbos, NA Mifsud, K Kedzierska
Immunology and Cell Biology | WILEY | Published : 2017
DOI: 10.1038/icb.2016.71
Abstract
Epstein-Barr virus (EBV) is one of the most common viruses in humans, capable of causing life-threatening infections and cancers in immunocompromised individuals. Although CD8 + T cells provide key protection against EBV, the persistence and dynamics of specific T-cell receptor (TCR) clones during immunosuppression in transplant patients is largely unknown. For the first time, we used a novel single-cell TCRαβ multiplex-nested reverse transcriptase PCR to dissect TCRαβ clonal diversity within GLCTLVAML (GLC)-specific CD8 + T cells in healthy individuals and immunocompromised lung transplant recipients. The GLC peptide presented by HLA-A∗02:01 is one of the most immunogenic T-cell targets fro..
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Grants
Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
We gratefully acknowledge the generous support of all the clinicians, nurses and allied health professionals associated with the Lung Transplant Service at The Alfred Hospital, especially the patients recruited for this study. KK is supported by the Australian National Health and Medical Research Council (NHMRC) Program Grant (ID 1071916) and is an NHMRC SRFB Fellow (ID1102792). EJG was a recipient of an NHMRC Aboriginal and Torres Strait Islander Health Research Scholarship and Douglas and Lola Douglas Scholarship in Medical Science and is supported by an NHMRC CJ Martin Fellowship.